Table 1.
Width of labeled region of extrasynaptic NMDARs using immunogold localization of NR1 in CA1 stratum radiatum of hippocampus, or DAB/immunoperoxidase of hippocampal neuronal cultures
Experiment* | Average in nm (N=) | Range in nm | Combined averages |
---|---|---|---|
Gold particles | 25 (77) | 5-93 | |
Flag-NR2A-in vitro | 227 (35) | 50-560 | |
Myc-NR2B-in vitro | 251 (65) | 51-768 | Transf. 2A+2B=243# |
NR2A-in vitro | 198 (50) | 51-538 | |
NR2B-in vitro | 186 (69) | 51-602 | Native 2A+2B=191# |
Gold particle clusters of 2-6 5 nm gold particles were counted from 13 experimental groups on 2 animals. Live surface NR2A or NR2B labeling was counted from 2 experiments.
p=0.00127 for transfected NR2 subunits versus native NR2 subunits.