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HMVECs (a) or HUVECs (b) had serum and growth factors removed for 1 h and were then stimulated with basal media containing 10 n M Fkn in basal media for the times indicated. Cell lysates were generated and 20 μg protein/lane was separated by PAGE and then blotted using standard Western blotting techniques. Phosphorylated Akt was compared with total Akt to assure equal loading. Blots are representative of three independent experiments.
