Fig. 5.

Cell surface-expression of co-stimulatory molecules CD40, CD54 (ICAM-1) and CD86 by Muc1^+/+ and Muc1^−/− primary lung DC as quantified by multi-parameter flow cytometry. Data are expressed (in a and b) as geometric mean fluorescence intensity (MFI) ± 1 standard deviation about the mean (n = 3 independent experiments of 6 mice per group). ∗ p < 0.05; ∗∗ p < 0.01 or p < 0.001; ⁺⁺ p < 0.01, all wild-type vs. knockout DC. c Typical sets of flow cytometric histograms of the data described in a and b as well as expression of cell surface MHC class II. FLA = Flagellin stimulated.