. 2009 Nov 2;2(2):123–143. doi: 10.1159/000254790

Table 1.

Cytokine secretion by co-cultures of lung or splenic DC-stimulated naive CD4+ T cells

	TNF			IL-12			IFN-γ			IL-13			IL-6
	R	L	F	R	L	F	R	L	F	R	L	F	R	L	F
Lung DC
WT	31.3 (10.7)	386.2 (96.3)^∗∗	348.9 (102)^∗∗	38.7 (9.6)	296.4 (42.8)^∗∗	227.7 (52.9)^∗∗	46.3 (9.6)	217.7 (66.3)^∗∗	474.9 (103)^∗∗	8.6 (10.2)	67.2 (34.5)^∗	121.1 (33.7)^∗	17.8 (9.6)	924.7 (213)^∗∗	533.6 (87.7)^∗∗
KO	89.3 (17.6)	1,352 (343)^{∗∗, +}	965.7 (261)^{∗∗, +}	53.3 (12.6)	198.4 (67.5)^∗	145.4 (58.1)^{∗, +}	145.8 (22.4)⁺	688.2 (189)^{∗∗, +}	928.8 (212)^{∗∗, +}	14.3 (9.6)	98.3 (42.9)^∗	102.4 (29.7)^∗	23.4 (8.7)	2,122 (403)^{∗∗, +}	1,044 (422)^{∗∗, +}

Splenic DC
WT	16.3 (5.8)	142.3 (36.9)^∗	157.6 (42.2)^∗	8.7 (5.5)	358.7 (74.8)^∗	295.3 (89.4)^∗	21.2 (19.7)	213.4 (43.7)^∗	169.8 (66.8)^∗	12.2 (7.4)	43.3 (19.6)^∗	102.8 (39.3)^∗	3.8 (8.5)	338.4 (76.9)^∗	197.4 (67.8)^∗
KO	29.3 (8.6)	523.8 (109)^{∗∗, +}	438.7 (133)^{∗∗, +}	10.1 (7.4)	176.6 (50.3)^{∗∗, +}	204.6 (33.6)^{∗∗, +}	92.2 (47.3)⁺	271.2 (62.6)^{∗∗, +}	439.1 (113)^{∗∗, +}	17.7 (11.2)	37.2 (19.3) NS	76.8 (30)^∗	34.2 (16.7)	1,077 (344)^{∗∗, +}	344.8 (108)^{∗∗, +}

In addition to assessing cytokine elaboration from a BM-DC and T cell co-culture system, we also studied the comparative secretion of the same cytokines (TNF-α, IL-12p40, IFN-γ and IL-13) as well as IL-6 by multiplex cytokine array analysis (see Materials and Methods) from primary lung or primary splenic Muc1^+/+ and Muc1^−/– DC. Data are pg/ml of the DC/T cell co-culture 81 standard deviation about the mean (shown in parentheses).

R = Resting DC that were unstimulated; L = LPS (100 ng/ml) stimulated cells; F = flagellin- (100 ng/ml) stimulated cells; NS = no statistically significant difference between stimulated and resting cells in the co-culture.

Alpha values: ^∗ p < 0.05; ^∗∗ p < 0.01; ⁺ p < 0.05, all wild-type vs. knockout DC (product of 3 independent experiments for lung and spleen DC).