Fig. 2.

PrgJ and FliC share sequence identity within an NLRC4 activating motif. (A) Alignment of FliC and PrgJ starting at the indicated amino acid through the carboxy terminal residue. Identity (black) and similarity (gray) are indicated. (B) 6xHis-tagged PrgJ or protein carrying the indicated mutations were purified and delivered to the cytosol of LPS primed BMM. IL-1β secretion was determined 1 h later. (C) CHO cells expressing TLR5 were stimulated with the indicated protein and expression of firefly luciferase under the control of NF-κB was measured.