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Kinetic parameters of the BcPMH Cys57Ala mutant for substrates 1b–6b
| C57A mutant |
Ratio (wt:C57A) | |||
| kcat (s-1) | KM (mM) | kcat/KM (M-1 s-1) | kcat/KM | |
| 1b | (3.1 ± 0.1) ∗ 10-5 | 1.16 ± 0.08 | (2.6 ± 0.1) ∗ 10-2 | 828 ± 70 |
| 2b | (5.6 ± 0.1) ∗ 10-3 | 0.71 ± 0.06 | 7.8 ± 0.7 | 1178 ± 131 |
| 3b | > 5.4 ∗ 10-6 | > 2.4 | (2.3 ± 0.1) ∗ 10-3 | 7.0 ± 0.3 |
| 4b | (1.90 ± 0.02) ∗ 10-3 | 0.061 ± 0.005 | 31 ± 3 | 467 ± 55 |
| 5b | (3.1 ± 0.2) ∗ 10-5 | 21 ± 5 | (1.5 ± 0.3) ∗ 10-3 | 405 ± 97 |
| 6b | (1.9 ± 0.2) ∗ 10-5 | 0.14 ± 0.03 | 0.13 ± 0.03 | 361 ± 95 |
