Fig. 2.
SIMS analysis of a strongly paired mated T. thermophila. (A) Differential interference contrast microscopy image of a mating cell pair. (Scale bar: 25 μm.) (B–D) SIMS images of a triturated pair of mating T. thermophila. C5H9 is mapped in (B), PC is mapped in (C), and 2-AEP is mapped in (D) (the intensity in the 2-AEP image has been multiplied by 3). (E) Region of interest analysis of the same cells. The red mass spectrum is from the cell bodies, and the green mass spectrum is from the junction. The Inset highlights the regions on the SIMS image, red for the cell bodies and green for the junction.