Fig. 4.

Mapping of tagged MatK binding sites on the trnK precursor. (A) Identification of trnK RNA segments associated with HA-tagged MatK in chloroplast stroma by RIP-Chip analysis. The enrichment ratios (F635:F532) were normalized between three assays for each genotype (C+, N+, C−, and N−). The background-corrected, median-normalized values for replicate spots from the tagged MatK assays were divided by those from the control assays and plotted according to their position within the trnK gene. Intron domains are marked below. (B) Enrichment values for control oligos corresponding to genes not identified in intial RIP-Chip experiments. B is based on the same set of experiments as described in A.