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. 2010 Jan 26;107(7):3012–3017. doi: 10.1073/pnas.0914902107

Fig. 4.

Fig. 4.

Poly (I:C) promotes kidney metalloproteinase activity and up-regulates expression of metalloproteinases 2 and 14 in a macrophage-dependent manner. Liposomal clodronate or PBS was injected i.v. at the onset of proteinuria (day 0) in poly (I:C)- treated mice. Injections were repeated after 3, 7, and 11 days of proteinuria. Untreated nonproteinuric, poly (I:C)-treated proteinuric, and poly (I:C) and liposomal clodronate treated proteinuric NZB/W mice, were analyzed after 14 days of proteinuria. (A) Renal cortex mRNA was analyzed by real time PCR; values are relative to expression of the gene encoding GAPDH (n = 4–6 mice per group). *, P < 0.05; **, P < 0.01. (B) Gel zymography of nonreduced kidney cortex lysates. Each lane represents different individual mice. (C) Immunoblot analysis of MMP9, MMP2, and MMP14 in kidney cortex lysates. Tubulin was used as loading control. (D) In situ zymography with a gelatin substrate on unfixed kidney cortex cryosections. Representative of three independent experiments.