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. 2010 Jan 26;107(7):3012–3017. doi: 10.1073/pnas.0914902107

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Fig. 6. — Poly (I:C)- induced kidney macrophages have a dysregulated tissue repair phenotype. (A) Whole kidney-cell suspensions were labeled with antibodies against lineage markers (B220, CD4, CD8, CD5, CD49b), as well as myeloid cell markers (CD11b, F4/80, CD11c) and analyzed by FACS. Lineage- (B220- CD4- CD8- CD5-CD49b-) CD11b^hi CD11c^intF4/80⁺ cells were sorted. (B) Total RNA from sorted kidney macrophages was analyzed by real-time PCR for IL10, TNFα, MMP2, MMP14, osteopontin, HB-EGF, and PDGF-C; values are relative to expression of the gene encoding GAPDH (n = 4–5 mice per group). *, P < 0.05.