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. 2010 Jan 26;107(7):3012–3017. doi: 10.1073/pnas.0914902107

Fig. 7.

Fig. 7.

Type I IFN-mediated induction of proliferative crescentic nephritis. Untreated nonproteinuric, untreated spontaneously proteinuric and AdIFNα-treated proteinuric NZB/W mice were analyzed after 14 days of proteinuria. (A) Paraffin-embedded kidney sections stained for basement membrane and matrix constituents with PAS stain. (B) Glomerular and interstitial histologic scores. Each symbol indicates an individual mouse (n = 3–4 mice per group). *, P < 0.05. (C) Whole-kidney single-cell suspensions were analyzed by FACS after 1, 4, and 14 days of proteinuria. Relative percentage of B cells, T cells, granulocytes, macrophages, and dendritic cells per total kidney cells. Percentages are shown as the means ± SD from three independent experiments (n = 3–4 mice per group). (D) In situ zymography with a gelatin substrate on unfixed kidney cortex cryosections. Representative of three independent experiments.