Fig. 2.

Uptake of Gly3P occurs through Git1 and Pho91 and is not required for activation of the PKA target trehalase. (A) Gly3P can serve as source of phosphate for a strain lacking all phosphate carriers. Growth of the wild type strain and the phoΔnull strain on SD medium (contains 7 mM phosphate) supplemented with different concentrations of Gly3P. (B) Uptake rate of ¹⁴C-Gly3P in phosphate-starved cells of the wild type, pho3Δ pho5Δ, git1Δ pho3Δ pho5Δ and git1Δ pho91Δ pho3Δ pho5Δ strains and of the phoΔnull strain overexpressing a single phosphate carrier. (C) Activation of the PKA target trehalase after addition of Gly3P to phosphate-starved cells does not require the Gly3P transporters Git1 and Pho91. Strains: wild type (•), git1Δ pho3Δ pho5Δ (○), pho91Δ pho3Δ pho5Δ (▴), git1Δ pho91Δ pho3Δ pho5Δ (Δ).