Table 1.

Oxidative kinetic resolution of ketones rac-1a–k using PAMO variant Gln93Asn/Pro94Asp under standard reaction conditions (Materials and Methods). Details of the procedure for expression, purification, and enzymatic activity assays are included in SI Material and Methods. Note that the R/S assignment may change in accordance with the Cahn-Ingold-Prelog convention; the absolute configuration is as shown in Scheme 1.

Entry	Substrate	Specificactivity (U/mg)	Conv. (%)	Residual ketone 1		Lactone 2		E-value
				ee (%)	Abs. conf.	ee (%)	Abs. conf.
1	rac-1a	64	45	38	S	95	R	92
2	rac-1b	40	17	2.7	S	18	R	1.5
3	rac-1c	35	15	46	R	91	S	25
4	rac-1d	49	21	25	R	95	S	50
5	rac-1e	42	37	37	R	95	S	68
6	rac-1f	51	36	14	R	92	S	40
7	rac-1g	24	23	19	S	94	R	42
8	rac-1h	39	15	17	S	> 99	R	> 200
9	rac-1i	48	42	43	S	86	R	25
10	rac-1j	58	43	58	S	98	R	> 200
11	rac-1k	52	11	17	S	96	R	55