Table 1.
Relative values of the various lymphoid populations in bone marrow and spleen
| n | WT | α1KI/α1KI | |
| Bone marrow | |||
| B cells (B220+) | 4/8 | 30.75 ± 4.04% (6.41 ± 2.95) | 12.26 ± 1.89%*** (2.15 ± 0.66) |
| Gated on B cells (B220+) | |||
| Pro-B cells (CD117+B220+) | 3/7 | 7.87 ± 2.25% (0.39 ± 0.21) | 24.88 ± 3.26%* (0.44 ± 0.16) |
| Pro-B/pre-B cells (CD43+B220+) | 4/8 | 33.90 ± 4.57% (2.33 ± 1.24) | 77.75 ± 3.32%* (1.55 ± 0.38) |
| Pre-B cells (CD25+B220+) | 4/8 | 36.94 ± 3.85% (2.16 ± 0.8) | 11.13 ± 1.73%*** (0.3 ± 0.15**) |
| Immature B cells (B220lowIgMlow) | 3/7 | 11.40 ± 3.59% (0.48 ± 0.2) | ND |
| Recirculating B cells (B220highIgMhigh) | 3/7 | 4.95 ± 1.85% (0.17 ± 0.06) | ND |
| Spleen | |||
| Macrophages (F480+CD11b+CD19−) | 3/5 | 3.52 ± 0.50% (2.20 ± 0.57) | 6.44 ± 0.71%* (2.66 ± 0.47) |
| NK (DX5+CD3−CD19−) | 3/5 | 3.91 ± 0.12% (2.36 ± 0.40) | 4.50 ± 0.52% (1.76 ± 0.18) |
| NKT (DX5+CD3+CD19−) | 3/5 | 1.05 ± 0.05% (0.64 ± 0.11) | 2.47 ± 0.36%* (1.03 ± 0.20) |
| T cells (DX5−CD3+CD19−) | 3/5 | 39.81 ± 2.49% (23.77 ± 3.45) | 48.94 ± 3.71% (19.90 ± 2.65) |
| B220+ cells | 7/7 | 34.07 ± 0.87% (22.17 ± 2.32) | 7.29 ± 1.08%*** (2.16 ± 0.47***) |
| Plasma cells CD138+ K intra | 7/7 | 0.50 ± 0.07% (0.31 ± 0.03) | 1.06 ± 0.23%* (0.34 ± 0.08) |
| Gated on B220+ cells | |||
| Resting B cells (IgD+IgM+B220+) | 7/7 | 90.66 ± 0.86% (20.15 ± 2.14) | ND |
| Transitional B cells (AA4.1+B220+) | 3/3 | 12.37 ± 2.6% (2.98 ± 0.75) | 12.97 ± 1.78% (0.26 ± 0.13*) |
| FO B cells (CD23+CD21+B220+) | 7/7 | 67.27 ± 3.72% (15.12 ± 1.92) | 30.31 ± 4.74%*** (0.66 ± 0.21***) |
| MZ B cells (CD23−/low CD21+B220+) | 7/7 | 13.16 ± 2.67% (2.86 ± 0.62) | 7.71 ± 2.29% (0.16 ± 0.05***) |
| B1 cells (CD5lowB220+) | 3/3 | 8.71 ± 0.05% (1.32 ± 0.11) | 13.94 ± 2.82% (0.33 ± 0.07*) |
| Non-B cells expressing B220 (B220+LC−) | 7/7 | 6.14 ± 0.96% (1.36 ± 0.28) | 34.01 ± 4.16%*** (0.70 ± 0.14) |
| Total B lymphocytes (B220+LC+) | 7/7 | 93.86 ± 0.96% (20.81 ± 2.19) | 65.99 ± 4.156%*** (1.46 ± 0.38***) |
| κ+ cells | 7/7 | 90.54 ± 1.12% (20.07 ± 2.12) | 63.24 ± 4.03%*** (1.4 ± 0.36***) |
| λ+ cells | 7/7 | 5.10 ± 0.26% (1.13 ± 0.15) | 1.77 ± 0.22%*** (0.036 ± 0.009***) |
| CD19+ B cells (B220+CD19+) | 7/7 | 95.46 ± 0.26% (21.15 ± 2.2) | 64.96 ± 5.41%*** (1.44 ± 0.36***) |
| B220+mIgM+ cells | 7/7 | 93.17 ± 0.14% (20.65 ± 2.2) | ND |
| B220+mIgA+ cells | 7/7 | ND | 62.23 ± 5.00% (1.39 ± 0.39) |
Second column indicates numbers (n) of WT/mutant animals used. Third and fourth columns indicate percentages of cells (and absolute values in millions of cells in parentheses). ND, not detectable. Asterisks indicate statistically significant differences between values observed in the group of α1KI/α1KI mice and the control group: *P < 0.05; **P < 0.01; ***P < 0.001.