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. 2010 Jan 26;107(7):3251–3256. doi: 10.1073/pnas.0913035107

Fig. 4.

Fig. 4.

The endosomal, but not the tonoplast V-ATPase is limiting under salt stress. (A) Inhibition of root growth through NaCl. The control condition was set to 100%. Error bars show SE with n = 16. Data are representative for one of three independent experiments. (B–D and F) HPLC analysis of sodium, chloride, potassium, and nitrate contents in 13-day-old seedlings grown on plates. Error bars represent SD of four biological replicates. (E) K+-stimulated PPase activity was determined from microsomal membranes of 4-day-old etiolated Arabidopsis seedlings. Control activity was set to 100%. Error bars represent SE of three independent experiments. (G) A RNAi against VHA-a1 was induced with 0.2% EtOH. Seedlings were grown for 15 days on vertical agar plates containing different concentrations of NaCl. The root length of the seedlings was measured (Fig. S7). (H) Inhibition of growth through salt upon EtOH induction. Differences between control samples (− EtOH) and treatment samples (+ EtOH) are expressed in percent.