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(A) Size exclusion chromatography. Recombinant Keap1 was purified using a Superdex S200 SEC column before EM analysis. Protein was routinely eluted at 1.03 mL as a single sharp peak; this fraction was used for EM image analysis. (B) SDS-PAGE and silver staining. The purity of fractions eluted from the SEC was monitored by SDS-PAGE and silver staining. (C) Purity of Keap1 was verified by silver staining (Left) and immunoblotting analysis using anti-Keap1 antibody (Right).
