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Analysis of reinnervation. (A–D) Photomicrographs of the NMJ in the tibialis muscle stained with bungarotoxin at 7 days after induction of ischemia and treatment with blank alginate gel (A), alginate gel delivering VEGF (B), alginate gel delivering IGF1 (C), or alginate gel delivering VEGF and IGF1 (V+I, D). (E) Quantification of the sites of overlap of motor neuron axon (yellow) and endplate (red) as a site of reinnervation. Values are mean ± SD. *P < 0.05.
