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. 2010 Feb 2;107(8):3582–3587. doi: 10.1073/pnas.0911908107

Fig. 4.

Fig. 4.

Genetic requirements for 26G-RNA expression. (A) Northern blot of siR26-1 in wild-type and mutant embryos. Input (Left) and ERGO-1 IP (Right) samples are indicated. Loading control: mir-66. (B) Northern blot of siR26-1 in RNAi mutant (adult). The membrane was first hybridized to a mir-66 probe as a loading control, which could not be removed completely, and is indicated by the asterisk (*). All lanes shown were from the same membrane and exposure. Only relevant lanes are shown. (C) Northern blot of siR26-263 in the indicated RdRP mutants (adult). Loading control: 21U-1. All lanes were from the same membrane and exposure. Only the relevant lanes are shown. (D) Northern blot of siR26-1 in mutants with multiple WAGO deletions (MAGO and MAGO+2) (9, 13). Loading control: mir-66. Adults were used. All lanes were from the same membrane and exposure. Only the relevant lanes are shown.