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. 2010 Feb 2;107(8):3517–3521. doi: 10.1073/pnas.0914723107

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Fig. 3. — Delays between individual docking and fusion events and SNAP25 dependence of the overall fusion rate. (A) Distribution of delay times, normalized to integrate to unity (t-LP = 10K, v-LP = 150, 32 °C, 175 delays from 8 acquisitions; bin width = 100 ms). (B) The same delays as in A, presented as a survivor function (SI Text). Inset shows the full span of the distribution, including a small fraction of delays (<15–20%) which occur on >1 s timescales. (C) Effect of the limited time resolution on the sampling of the true delays. The mean delay for fast fusions (delays s) versus acquisition period T_acq. The mean delay extrapolated to T_acq = 0 ms is ms. (D) Comparison of fusion rates between SBLs reconstituted with Syx·SNAP25 (190 fusions, 7 acquisitions, t-LP = 10K) and with Syx alone (60 fusions in 19 acquisitions, t-LP = 10K). SBLs were formed from t-SUVs reconstituted side by side. Identical v-SUV preparations were used. Omission of SNAP25 resulted in a 12-fold reduction in the normalized fusion rate (v-LP = 200, T = 30–32 °C).

Inline graphic — Delays between individual docking and fusion events and SNAP25 dependence of the overall fusion rate. (A) Distribution of delay times, normalized to integrate to unity (t-LP = 10K, v-LP = 150, 32 °C, 175 delays from 8 acquisitions; bin width = 100 ms). (B) The same delays as in A, presented as a survivor function (SI Text). Inset shows the full span of the distribution, including a small fraction of delays (<15–20%) which occur on >1 s timescales. (C) Effect of the limited time resolution on the sampling of the true delays. The mean delay for fast fusions (delays s) versus acquisition period T_acq. The mean delay extrapolated to T_acq = 0 ms is ms. (D) Comparison of fusion rates between SBLs reconstituted with Syx·SNAP25 (190 fusions, 7 acquisitions, t-LP = 10K) and with Syx alone (60 fusions in 19 acquisitions, t-LP = 10K). SBLs were formed from t-SUVs reconstituted side by side. Identical v-SUV preparations were used. Omission of SNAP25 resulted in a 12-fold reduction in the normalized fusion rate (v-LP = 200, T = 30–32 °C).