Figure 6.

Confocal images of macrophage uptake of modified LDL under hypoxic or normoxic conditions. RAW and HARM cells grown to subconfluent state were exposed to either hypoxia or normoxia for 24 hr. Cells were then incubated with acetylated-LDL conjugated with a fluorescent probe (DiI-Ac-LDL) for an additional 4 hr. A confocal scanning laser microscopy imaging system was used to examine macrophage uptake of modified LDL. The intensity of fluorescence represents the level of modified-LDL uptake by cells.