Fig. 3.

Cellular targets of the Env-mediated immunosuppression effect assayed by immune cell depletions in vivo. Serum viral loads (Lower) after injection of WT (○ and •) or DM (□ and ▪) F-MLV were measured in untreated (• and ▪) or NK.1.1-depleted (○ and □) Swiss (A) or Swiss-Nude (B) mice or in CD8-depleted (• and ▪) or CD8-depleted plus NK1.1-depleted (○ and □) Swiss mice (C). The data correspond to the mean ± SD for five mice and are representative of two to seven independent experiments. Cell depletions (duration of antibody treatment indicated with a bar; Materials and Methods) were controlled by flow cytometry on blood samples (Upper) , with numbers in the dot plot corners indicating the percentage of labeled cells in the corresponding quadrant. NK cell recovery after the last antibody injection was 50% 5 days later and >90% at day 8.