Fig. 4.

Protein expression of TRPV6, calbindin-D_9K and PMCA1 in placentas of WT and P0 fetuses at E17 and E19. Representative blots show expression within four paired litters. n = 8 litters at E17 and n = 6 litters at E19, except for calbindin-D_9K at E19, where n = 8 litters. β-actin was used as a marker of equal loading between samples and a single blot that has been stripped and reprobed for β-actin is shown. Arrows denote size of placental immunoreactive proteins. Protein loading was as follows: TRPV6, 20 μg placental membrane; calbindin-D_9K, 50 μg postnuclear supernatant at E17, 20 μg at E19; PMCA1, 40 μg placental membrane. mAM and RB denote mouse placental apical membrane (derived from maternal facing-plasma membrane of syncytiotrophoblast layer II) and rat brain, respectively, included as positive controls. Graphs show densitometry values for TRPV6 (A), calbindin-D_9K (B), and PMCA1 (C) at both E17 and E19. Each symbol corresponds to densitometry values expressed as a ratio of P0/WT within a single litter. Solid line denotes median values for all of the litters shown at each gestational age. Dotted line represents ratio of 1 (no difference between P0 and WT). **, P < 0.01, Wilcoxon signed-rank test.