Fig. 3.

Identification of global LGI1-containing protein complex in the brain. (A) In vivo LGI1-associated protein complex purified from the LGI1-FH–expressing mouse (Tg1), including p100, 90, 80, 65, 55, and 30 (arrowheads, from Top). (B) Major constituents of LGI1 complex contained ADAM23 as well as ADAM22 and PSD-95. Closed arrowheads, LGI1-FH; asterisk, nonspecific band; open arrowhead, endogenous LGI1; Ft, flow through; Elu, elution; Res, resin after elution. (C) More than 90% of expressed LGI1-FH were isolated by TAP. (D) LGI1-associated protein network analyzed by shotgun mass spectrometry. The identified specific proteins were lined up according to the SEQUEST score that is used for protein identification and semiquantification. Error bars, ±SD (n = 3).