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. 2010 Mar 15;2010:734898. doi: 10.1155/2010/734898

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Copyright © 2010 María Esther Herrera-Aguirre et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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EhODP1 was localized in an EhkO-enriched fraction. (a) EhkOs were purified by differential centrifugation and through a Nycodenz discontinuous gradient from exponentially growing trophozoites labeled with [³H]-Thymidine. Then, fractions were collected and their radioactivity content was measured. Data in graph represents total radioactivity incorporated in DNA within each fraction. (b) Western blot of proteins separated by 10% SDS-PAGE using rat anti-rEhODP1 antibodies. Lane 1, prestained molecular weight standards; lane 2, fresh total trophozoite extracts; lane 3, frozen total trophozoite extracts; lane 4, EhkOs-enriched fraction corresponding to fraction 9 in (a); lane 5, preimmune serum used with fresh total trophozoite extracts. Arrow indicates the 150 kDa polypeptide. Arrowhead shows the 70 kDa band.