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Site directed spin labeling of KcsA. (A) Reaction of the methanethiosulfonate reagent with cysteine to generate the side chain designated R1. (B) Labeled sites in KcsA. A KcsA monomer is shown as a grey ribbon with the rest of the channel depicted as a van der Waals surface. The side chains of labeled sites are depicted as orange sticks and van der Waals surfaces.
