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. 2010 Jan 7;151(3):1002–1009. doi: 10.1210/en.2009-0853

Figure 4.

Figure 4

Characterization of AgRP immunoactivity by HPLC in CSF pools from OVX monkeys before (left) and after (right) E2 replacement. AgRP immunoactivity in the eluted fractions was assayed by RIA. Arrows indicate elution positions of synthetic AgRP (83-132)-NH2 and of FL AgRP. Two prominent peaks of immunoactivity were found in CSF, eluting in the positions of both AgRP (83-132) and FL AgRP. The relative amount of AgRP (83-132) compared with FL AgRP was less in the CSF of E2-treated animals.