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. Author manuscript; available in PMC: 2010 Mar 17.
Published in final edited form as: Science. 2009 Jun 26;324(5935):1713–1716. doi: 10.1126/science.1171721

Figure 3. Mitochondrial STAT3 augments electron transport chain activity.

Figure 3

(A) Mitochondrial membrane potential measured with TMRE and recorded as mean fluorescence intensity (MFI) by flow cytometry in wild type (WT) or STAT3-deficient (KO) cells with or without H-RasV12. (B) Mitochondrial membrane potential of H-RasV12-expressing cells with wild type (WT), vector (KO), mitochondrially-targeted wild type (MTS-WT) or S727A-mutant STAT3. Activities of electron transport chain complexes II (C) and V (D) compared between H-RasV12-expressing wild type (WT) or STAT3-deficient (KO) cells. Unit activity of individual complexes normalized to citrate synthase activity from equivalent numbers of mitochondria. Statistical significance with p value <0.05* or <0.001*** by student’s t-test. (E) Lactate dehydrogenase activity in wild type (WT), STAT3-deficient (KO), and H-RasV12-expressing wild type (WT), STAT3-deficient (KO), mitochondrially-targeted wild type (MTS-WT), or mutant STAT3 (MTS-S/A) expressing cells. Results are means of 3 replicates. Error bars indicate SD.