Table 2.

Comparison of lymphocyte subset frequencies obtained using a statistical mixture model and traditional gating by experts. Values shown for each subset are percentages of total lymphocyte events. The CD3+ row is the mean value across all 4 preparations. Frequencies obtained using statistical mixture modeling have a clear background, while pooled frequencies by experts using traditional gating have a light grey background. Values in brackets are the lower and upper quartiles estimated by the group of experts. Samples S1 to S4 are quadruplicate samples from the same donor, sample T1 comes from a different donor. The suffix “A” indicates results obtained using the Automated algorithm, while “E” indicates Expert results.

Sample	S1A	S1E	S2A	S2E	S3A	S3E	S4A	S4E	T1A	T1E
CD3+	75.03	73 (68.3–74)	75.90	74	76.96	74	75.67	74	79.13	79 (78–79)
CD3+CD4+	23.65	23 (22–23.8)	23.31	23	23.94	23	23.01	24	26.26	26 (25–26)
CD3+CD8+	49.23	48 (45–49)	53.12	49	52.49	50	52.20	50	51.49	51 (49–51)
CD3−CD19+	16.19	16 (15.3–21.8)	16.89	16	15.93	16	15.48	14	10.83	11 (10–11)
CD3−CD(16+56)+	9.49	9 (8–10)	9.80	8	7.93	8	10.54	10	8.64	10 (9–11)