Figure 6.
The presence of GMI-CCAP in LG is necessary for CCAP to gate out the GPR prolongation of the gastric mill retractor phase. A, During saline superfusion, GPR stimulation selectively prolonged the retractor phase of the MCN1–gastric mill rhythm. Most hyperpolarized Vm, −71 mV. B, During CCAP superfusion, the effect of GPR stimulation on the retractor phase was gated out, despite stimulating GPR during successive retractor phases. Most hyperpolarized Vm, −74 mV. C, During CCAP superfusion, dynamic-clamp-mediated injection IMI-CCAP into LG using a negative version of GMI-CCAP (note hyperpolarizing current injections) eliminated the ability of CCAP to gate out the GPR action on the MCN1–gastric mill rhythm. Most hyperpolarized Vm, −74 mV. All three panels are from the same LG recording.