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. 2010 Feb 4;24(3):644–656. doi: 10.1210/me.2009-0357

Figure 3.

Figure 3

Impact of Ad-Cre infection on GH signaling does not reflect global effects on cell integrity or other pathways. A, Adenoviral infection of nonfloxed primary osteoblasts. Primary osteoblasts from mice without floxed IGF-IR genes were serum starved and treated with GH at the indicated concentrations for 10 min, after which detergent extracts were resolved by SDS-PAGE and sequentially immunoblotted with the indicated antibodies. Note the lack of effect of Ad-Cre vs. Ad-GFP on GH-induced signaling. The blots shown are representative of two independent experiments. B, Ad-Cre-mediated IGF-IR deletion did not affect PDGF signaling. IGF-IR-floxed primary osteoblasts were infected with Ad-GFP vs. Ad-Cre, as in Figs. 1 and 2. Serum-starved cells were treated with PDGF at the indicated concentrations for 10 min. Detergent extracts were resolved by SDS-PAGE and sequentially immunoblotted with anti-pAkt, anti-pERK, and anti-IGF-IRβ. The blots shown are representative of two independent experiments.