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. 2010 Jan 8;95(2):529–536. doi: 10.1210/jc.2009-1304

Figure 4.

Figure 4

Haplotype analysis of the variant band. An RT-PCR from exons 4–10 (primers 6F-10R) including the common c.919A/G polymorphism on exon 10 was performed from patients FR-1 and FR-64 who are heterozygous for this SNP. The normal and variant bands were isolated and sequenced. The location of the c.919A/G SNP is indicated with a star above the sequence. The top panel shows the sequence of exon 10 of the variant band. The middle panel shows the normal band. The bottom panel shows a genomic PCR of exon 10 to demonstrate that the patient was heterozygous. The variant band originated exclusively from one of the two alleles. The normal band originated from both. The graph depicts the preferential production of the variant band from one of the two FSHR alleles.