Figure 5.

Gene expression and nucleosome occupancy at different time points after nitrogen depletion and treatment with Cd²⁺. Cells were grown in EMM and starved for nitrogen or treated with 0.5 mM CdSO₄, and samples were taken out for RNA preparation or ChIP at time points indicated. (A) Gene expression monitored by real-time PCR and normalized to expression of dis2⁺ and to the signal obtained for EMM media. Data are represented as mean + SD. (B) ChIP analysis of H3 association with the open reading frame of indicated genes measured by real-time PCR. Data were normalized to the input (nonimmunoprecipitated) material and the signal obtained for EMM media. Data are represented as mean + SD.