Figure 2.

Specificity of CD spectral shifts with Hsp104 and rPrP. Predicted (−−), actual (—), and individual spectra as in Fig. 1. rPrP was prepared and folded into either β-sheet or α-helical forms. (A) Hsp104 and rPrPβ. (B) GroEL and rPrPβ. (C) Aldolase and rPrPβ. (D) Hsp104 and rPrPα. Proteins and rPrP were mixed (each at 0.5 mg/ml) with each other in LSB2. Reactions were incubated at 37°C for 1 hr, diluted 5-fold with cold water, and spectra were measured at 12°C.