Table 1.

ATP affects rate of CD change

Time, min	− ATP	+ ATP
0	2.9	2.2
3	7.9	15.2
6	9.9	17.2
10	10.9	18.2
15	11.8	18.8
20	12.4	19.2
30	13.3	19.8
72	16.2	21.9

The difference between the actual spectrum and the predicted spectrum at 225 nm for each time point is presented in millidegrees. In each of three separate experiments, the spectral change in mixtures of Sup35 and Hsp104 proceeded more rapidly with ATP than without ATP, although the absolute rates varied, most likely due to differences in the Sup35 preparations.