Table 2.
Effects of proteins and peptides on the ATPase activity of Hsp104
| HSB | LSB | |
|---|---|---|
| Hsp104 alone | 1.0 ± 0.05 | 1.0 ± 0.1 |
| Sup35 | 1.2 ± 0.1 | 0.6 ± 0.1 |
| N-term Sup35 | 1.2 ± 0.1 | 0.7 ± 0.1 |
| PrPβ | 1.2 ± 0.1 | 0.6 ± 0.05 |
| β-Amyloid 1-42 | 0.8 ± 0.05 | 0.3 ± 0.05 |
| β-Amyloid 1-40 | 1.1 ± 0.05 | 0.5 ± 0.1 |
| Reverse amyloid 40-1 | 1.1 ± 0.1 | 0.8 ± 0.1 |
| Aldolase | 1.1 ± 0.05 | 1.0 ± 0.1 |
| BSA | 1.0 ± 0.05 | 1.0 ± 0.05 |
| Apoferritin | 1.0 ± 0.1 | 1.0 ± 0.05 |
| IgM | 1.1 ± 0.05 | 1.1 ± 0.05 |
Hsp104 ATPase activity was measured in HSB or LSB1 and is presented as the activity of Hsp104 with protein divided by the activity of Hsp104 in buffer alone. Within individual experiments very little variance was observed; however, even with the results from three different preparations of Sup35 averaged here, only ≈10% variability was observed.