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Rap1 reverts the T4-2 malignant phenotype through different signaling pathways from those targeted by EGFR or β1-integrin. Activity and expression of the components of (A) EGFR-MAPK and (B) PTEN/Akt pathways were examined by Western blotting. C, the levels of FOXO1 protein and mRNA were determined by Western and reverse transcription-PCR analyses, respectively.
