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. Author manuscript; available in PMC: 2011 Mar 16.
Published in final edited form as: Dev Cell. 2010 Mar 16;18(3):450–462. doi: 10.1016/j.devcel.2009.12.023

Figure 4.

Figure 4

The mechanisms by which the BCL6/BCoR complex blocks Notch-dependent transcription.

A: HA-tagged Su(H), Flag-tagged MAM1 or/and BCL6 was expressed in embryos and protein extracts were isolated from 50 embryos at stage 10. Co-immunoprecipitation with α-Notch antibody was performed. B: Co-immunoprecipitation using in vitro synthesized proteins. C, D: Flag-tagged MAM1, Flag-tagged dn-MAM1, BCL6 or/and Myc-tagged NBD-S was expressed in embryos and protein extracts were isolated from 50 embryos at stage 10 for each experiment. E, F: Myc-tagged BCoR was expressed in embryos without (E) or with (F) the BCL6 MO, and protein extracts were isolated from 50 embryos at stage 10. Co-immunoprecipitation with α-Notch antibody was performed. α-vimentin antibody was used for a mock immunoprecipitation. G: The expression of Pitx2 at stage 25 was tested by whole mount in situ hybridization and the ratios of Pitx2-expressing embryo number versus total tested embryo number are shown. Total numbers of each injection are shown as “n” on the top of each bar.