Skip to main content
. Author manuscript; available in PMC: 2011 Mar 16.
Published in final edited form as: Dev Cell. 2010 Mar 16;18(3):450–462. doi: 10.1016/j.devcel.2009.12.023

Figure 5.

Figure 5

ESR1 is a target of BCL6 during LR patterning and neural development.

A: BCL6 MO was injected into the left side of embryos and left LPM tissues were dissected from 20 embryos at stage 25 for quantitative RT-PCR. The injection side was traced by the co-injection of GFP (data not shown). * P<0.05, n=3. B: BCL6 MO was injected into a dorsal blastomere of four-cell stage embryos and embryos were fixed at stage 14. Increased ESR1 expression and decreased N-tubulin expressions are indicated by white and black arrows, respectively. C: BCL6 MO or/and Notch1 MO was injected into the left side of embryos and left LPM tissues were dissected from 20 embryos at stage 25 for quantitative RT-PCR. * P<0.01, n=3, **P<0.01, n=3, ***P<0.01, n=3. D: 1 ng ESR1-GR or Hairy2-GR was injected for each experiment. E: 40 ng ESR1 MO or/and BCL6 MO was injected for each experiment. The injected side is indicated by L (left) beside the names of injected samples. L: left, R: right, a: anterior, p: posterior.