Table. Laterality scoringa in BCL6 MO, NBD-S or Notch1 MO injection.
Injection | Injection Side | Cardiac Orientation, Forward | Cardiac Orientation, Reverse | |||||||
---|---|---|---|---|---|---|---|---|---|---|
Gut Origin Right | Gut Origin Left | Gut Origin Right | Gut Origin Left | |||||||
CCWb | CWc | CCW | CW | CCW | CW | CCW | CW | n | ||
uninjected | 95.4 | 2.3 | 0.8 | 1.5 | 0.0 | 0.0 | 0.0 | 0.0 | 130 | |
<BCL6 MO> | ||||||||||
40 ng Control MO | Left | 96.1 | 1.3 | 2.6 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 76 |
20 ng Control MO | Left | 97.8 | 1.1 | 1.1 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 89 |
40 ng BCL6 MO | Right | 90.5 | 1.9 | 1.9 | 1.0 | 1.0 | 0.0 | 1.9 | 1.9 | 105 |
20 ng BCL6 MO | Right | 90.7 | 1.9 | 3.7 | 0.9 | 0.9 | 0.0 | 0.9 | 0.9 | 108 |
40 ng BCL6 MO | Left | 47.0 | 11.3 | 7.8 | 9.6 | 7.0 | 7.0 | 4.3 | 6.1 | 115 |
20 ng BCL6 MO | Left | 59.5 | 20.2 | 6.0 | 9.5 | 1.2 | 2.4 | 0.0 | 1.2 | 84 |
40 ng BCL6 MO/2 ng mBCL6-GR | Left | 89.4 | 4.3 | 1.1 | 2.1 | 1.1 | 1.1 | 1.1 | 0.0 | 94 |
20 ng BCL6 MO/2 ng mBCL6-GR | Left | 85.7 | 3.1 | 2.0 | 1.0 | 1.0 | 2.0 | 2.0 | 3.1 | 98 |
<NBD-S> | ||||||||||
1 ng NBD-S-GR | Left | 70.0 | 7.0 | 6.0 | 3.0 | 5.0 | 2.0 | 4.0 | 3.0 | 100 |
2 ng NBD-S-GR | Left | 55.0 | 11.0 | 9.0 | 4.0 | 4.0 | 3.0 | 8.0 | 4.0 | 98 |
<Notch1 MO> | ||||||||||
150 ng Control MO | Left | 83.7 | 2.4 | 8.1 | 2.4 | 0.8 | 0.8 | 0.8 | 0.8 | 123 |
80 ng Control MO | Left | 91.3 | 3.6 | 2.2 | 2.9 | 0.0 | 0.0 | 0.0 | 0.0 | 138 |
150 ng Notch1 MO | Left | 38.7 | 33.1 | 8.9 | 6.5 | 2.4 | 4.0 | 3.2 | 3.2 | 124 |
80 ng Notch1 MO | Left | 57.1 | 22.7 | 4.2 | 1.7 | 3.4 | 3.4 | 4.2 | 3.4 | 119 |
Lateral scoring according to Branford et al. (2000).
Numbers indicate percentage of embryos displaying phenotype (total embryos as n)
CCW: counterclockwise
CW: clockwise