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. Author manuscript; available in PMC: 2011 Mar 16.
Published in final edited form as: Dev Cell. 2010 Mar 16;18(3):450–462. doi: 10.1016/j.devcel.2009.12.023

Table. Laterality scoringa in BCL6 MO, NBD-S or Notch1 MO injection.

Injection Injection Side Cardiac Orientation, Forward Cardiac Orientation, Reverse
Gut Origin Right Gut Origin Left Gut Origin Right Gut Origin Left
CCWb CWc CCW CW CCW CW CCW CW n
uninjected 95.4 2.3 0.8 1.5 0.0 0.0 0.0 0.0 130
<BCL6 MO>
40 ng Control MO Left 96.1 1.3 2.6 0.0 0.0 0.0 0.0 0.0 76
20 ng Control MO Left 97.8 1.1 1.1 0.0 0.0 0.0 0.0 0.0 89
40 ng BCL6 MO Right 90.5 1.9 1.9 1.0 1.0 0.0 1.9 1.9 105
20 ng BCL6 MO Right 90.7 1.9 3.7 0.9 0.9 0.0 0.9 0.9 108
40 ng BCL6 MO Left 47.0 11.3 7.8 9.6 7.0 7.0 4.3 6.1 115
20 ng BCL6 MO Left 59.5 20.2 6.0 9.5 1.2 2.4 0.0 1.2 84
40 ng BCL6 MO/2 ng mBCL6-GR Left 89.4 4.3 1.1 2.1 1.1 1.1 1.1 0.0 94
20 ng BCL6 MO/2 ng mBCL6-GR Left 85.7 3.1 2.0 1.0 1.0 2.0 2.0 3.1 98
<NBD-S>
1 ng NBD-S-GR Left 70.0 7.0 6.0 3.0 5.0 2.0 4.0 3.0 100
2 ng NBD-S-GR Left 55.0 11.0 9.0 4.0 4.0 3.0 8.0 4.0 98
<Notch1 MO>
150 ng Control MO Left 83.7 2.4 8.1 2.4 0.8 0.8 0.8 0.8 123
 80 ng Control MO Left 91.3 3.6 2.2 2.9 0.0 0.0 0.0 0.0 138
150 ng Notch1 MO Left 38.7 33.1 8.9 6.5 2.4 4.0 3.2 3.2 124
 80 ng Notch1 MO Left 57.1 22.7 4.2 1.7 3.4 3.4 4.2 3.4 119
a)

Lateral scoring according to Branford et al. (2000).

b)

Numbers indicate percentage of embryos displaying phenotype (total embryos as n)

c)

CCW: counterclockwise

d)

CW: clockwise