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. Author manuscript; available in PMC: 2011 Mar 16.
Published in final edited form as: Dev Cell. 2010 Mar 16;18(3):410–424. doi: 10.1016/j.devcel.2009.12.022

Figure 2. Ofd1 localizes to the distal ends of mother, daughter, and procentrioles.

Figure 2

(A–C) Representative micrographs of WT and Ofd1Gt cells showing centrosomes (γ-tubulin), centrioles and cilia (acetylated tubulin), DNA (DAPI), and other indicated antibodies. (D) WT cells showing centrosomes (γ-tubulin), Ofd1, and the proximal procentriole (Sas-6). (E) Ofd1Ofd1myc cells showing centrosomes (γ-tubulin), Myc (Ofd1-Myc), and the distal procentriole (Poc5). Poc5 localizes more strongly to mother or daughter centrioles than to procentrioles. (F) Ofd1Ofd1myc cells showing centrosomes (γ-tubulin), Myc (Ofd1-Myc), and the distal centriole and procentriole (CP110). (G) Ofd1Ofd1myc cells showing centrosomes (γ-tubulin), Myc (Ofd1-Myc), and the proximal centriole (Rootletin). (H) Ofd1Ofd1myc cells showing centrosomes (γ-tubulin), Myc (Ofd1-Myc), and mother centriole subdistal appendages (Ninein). The mother centriole is marked by 3 Ninein foci (2 on the subdistal appendages and one on the proximal end) whereas the daughter centriole is marked by one Ninein focus (on the proximal end). (I) Ofd1Ofd1myc cells showing centrosomes (γ-tubulin), Myc (Ofd1-Myc), and mother centriole appendages (Odf2). (J) Ofd1Ofd1myc cells showing Myc (Ofd1-Myc), mother centriole distal appendages (Cep164), and centrosomes (γ-tubulin). (K) – (L) Schematics showing Ofd1Ofd1myc cells stained for Myc (Ofd1-Myc), mother centriole subdistal (Ninein) or distal (Cep164) appendages, and centrosomes (γ-tubulin). MC, mother centriole. DC, daughter centriole. PC, procentriole. Scale bars (A)–(B) indicate 5 μm and 1 μm (inset), and 1 μm for (C)–(L). See also Figure S2.