Figure 2.

JDP2 expression does not alter liver pathophysiology. A. Immunohistochemistry of liver sections derived from either wild type (wt) or JDP2-transgenic (tg) mice. The expression of HA-JDP2 transgene was analyzed with anti-JDP2 antibody (left panel). The extent of apoptotic and proliferation of hepatocytes was analysed by TUNEL assay (middle panel) and PCNA staining (right panel). Arrows indicate positively stained cells. Liver/body weight ratio (B.) and Serum ALT (C.) was determined from one month old of either wild type control (wt, black bar) or JDP2-transgenic (tg, grey bar) mice. The results represent the average and SEM of the indicated number of animals (n). D. Real time qPCR of the indicated selected genes was performed from cDNA derived from mRNA extracted from liver of wild type and JDP2-transgenic mice. The results represent the calculated expression ratio between JDP-transgenic divided by the expression of the corresponding gene of the wild type mice. The average and SEM of three different experiments performed with pooled mRNA from at least three mice is presented.