Figure 7.

JDP2-tg and liver cancer in 14 days DEN protocol. Mice were injected with DEN 25 mg/Kg at 14 days postnatal and sacrificed at eleven months of age. A. Schematic diagram representing the 14 day DEN protocol and doxycycline treatments used in the indicated experimental groups. Mice were treated with doxycycline 0.2 mg/ml dissolved in 5% sucrose in the drinking water as indicated in the scheme (black line, no JDP2 expression). Grey line represents JDP2 expression. Serum ALT (B.) and Liver/body weight ratio (C.) was determined. The results represent the average and SEM of the indicated number of animals (n). D. The number of macroscopic tumors in liver derived from eleven months old mice injected with DEN and treated as depicted schematically for each experimental group. Wild type (wt, black bar) and JDP2-transgenic (tg). The results represent the average and SEM of the number (n) of animals in each group. Asterisks represent P values < 0.05