Figure 6.
Total ErbB and phosphorylated ErbB (activation) receptor levels in neoplastic +SA mammary epithelial cells. +SA mammary tumor cells were initially plated a density of 1 × 106 cells/100 mm culture dish. Afterwards, cells were incubated overnight in mitogen-free media. Cells in all treatment groups were then stimulated with 100 ng/ml EGF for 0-60 min. Following treatment exposure, whole cell lysates were prepared and then subjected to PAGE followed by Western blot analysis for total and phosphorylated (active) ErbB receptor levels. Each Western blot is a representative image of data obtained for experiments that were repeated at least three times.