Figure 4. Silencing of PARM-1 augmented apoptotic response to ER stress.

A: Cultured neonatal rat cardiac myocytes were transfected with 30 nmol/l of three different siRNA duplexes, and assessed for PARM-1 expression 24 hours after transfection. B: Cells were treated with TG or TM 72 hours after transfection with siRNAs for 24 hours. Apoptotic cell death was assessed by TUNEL assay, and cell viability was analyzed by WST-8 assay. *P<0.05 versus control siRNA (Si cont).