Table 1.
Primer sequences and reaction conditions used in the PCR amplification of the various cDNAs
| Gene | Sense primer (5'-3') | Anti-sense primer (5'-3') | Added cDNA (ng) |
No. of cycles |
Gene bank accession N° | Product size (pb) | Ref. |
|---|---|---|---|---|---|---|---|
| IGF I | AAAATCAGCAGTCTTCCAAC | AGATCACAGCTCCGGAAGCA | 50 | 25 | [GenBank:X06108] | 299 | [49] |
| S16 | TCCAAGGGTCCGCTGCAGTC | CGTTCACCTTGATGAGCCCATT | 100 | 22 | [GenBank:XM_341815] | 100 | [50] |
| IGFBP3 | GCCGCGGGCTCTGCGTCAACGC | CTGGGACTCAGCACATTGAGGAAC | 200 | 25 | [GenBank:NM_012588] | 415 | [49] |
| IGFBP5 | TTGCCTCAACGAAAAGAGC | AGAATCCTTTGCGGTCACA | 100 | 25 | [GenBank:NM_012817] | 377 | [49] |
| GHR | GAGGAGGTGAACACCATCTTGGGC | ACCACCTGCTGGTGTAATGTC | 100 | 25 | [GenBank:J04811] | 534 | [50] |
| ERα | AATTCTGACAATCGACGCCAG | GTGCTTCAACATTCTCCCTCCTC | 200 | 25 | [GenBank:X061098] | 344 | [50] |
| ERβ | AAAGCCAAGAGAAACGGTGGGCAT | GCCAATCATGTGCACCAGTTCCT | 100 | 25 | [GenBank:U57439] | 352 | [50] |
| PRLLong | AAAGTATCTTGTCCAGACTCGCTG | AGCAGTTCTTCAGACTTGCCCTT | 100 | 30 | [GenBank:M74152] | 279 | [50] |
| IGFIR | TCCACCATAGACTGGTCTCT | ACGAAGCCATCTGAGTCACT | 50 | 30 | [GenBank:L29232] | 433 | [50] |
| P450arom | TGCACAGGCTCGAGTATTTCC | ATTTCCACAATGGGGCTGTCC | 100 | 30 | [GenBank:M33986] | 271 | [29] |