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Expression of eight alleles of P. falciparum PPPK-DHPS in E. coli. Eight different alleles of PPPK-DHPS were cloned into the pTrc vector and transformed into E. coli. Extracted proteins were separated by SDS/PAGE and stained with Coomassie blue (A) or transferred to nitrocellulose and probed with affinity-purified anti-DHPS antibodies (B). The order of the tracks in each panel from left to right is pTrc, D10-C, 3D7-C, Tak9/96-C, K1-C, W2 mef-C, PR145, D10-C/G581, and 3D7-C/E540.
