Purified recombinant PPPK-DHPS from eight different alleles. The eight alleles of PPPK-DHPS were purified using the protocol described in Material and Methods, and aliquots were separated by SDS/PAGE and either stained with Coomassie blue (A) or electroblotted to nitrocellulose and probed with the anti-DHPS antibodies (B). In both panels the tracks contain the sample as indicated and include, in order, D10-C, 3D7-C, Tak9/96-C, K1-C, W2 mef-C, PR145-C, D10-C/G581, and 3D7-C/E540.