Table 2.

Kinetic parameters of the PPPK-DHPS enzymes

Enzyme	Km, nM	Ki, μM	Vmax, mU/mg protein	kcat, s−1	kcat/Km, s−1M−1 × 106
D10-C	16.4 ± 1.3	0.14 ± 0.01	17.2 ± 0.3	0.024	1.46
3D7-C	24.2 ± 3.3	1.39 ± 0.23	29.8 ± 1.0	0.042	1.71
Tak9/96-C	88.9 ± 9.1	19.9 ± 4.0	33.0 ± 0.9	0.046	0.52
K1-C	77.4 ± 7.2	16.2 ± 2.7	27.6 ± 0.7	0.038	0.50
W2mef-C	193.3 ± 13.9	112 ± 10	33.7 ± 0.8	0.047	0.24
PR145-C	112.8 ± 5.8	98.3 ± 7.5	34.4 ± 0.5	0.048	0.42
D10-C/G581	20.0 ± 2.3	0.55 ± 0.06	32.4 ± 0.9	0.045	2.25
3D7-C/E540	105 ± 10	27.7 ± 6.1	41.4 ± 1.1	0.058	0.55

mU, milliunits.