Fig. 4.

Morphological features of the branched structures. a, b H & E staining of the branched structures in respectively 5 and 7 days deligated glands, characterized by short ducts (arrows) ending with acini (arrowheads). c Typical embryonic (e20) branched structures. d, e AB/PAS of 5 and 7 days deligated gland respectively, showing presence of glycoprotein in the acini at the end of the branching (arrows). Smooth muscle actin immunohistochemistry of 5 (f) and 7 days (g) deligated showing presence of myoepithelial cells around the acini (arrowheads) and the acinar-duct junction (arrows); sections were counterstained with haematoxylin. Fig. g shows background staining in few nuclei.