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Phosphorylation of mammalian cell substrates. (A) SYF cells were transfected with empty vector (Flag), human c-Src, MbSrc1, full-length MbSrc4, or MbSrc4-32K. Lysates were analyzed by SDS–PAGE and anti-pTyr Western blotting. The blots were reprobed with anti-Flag antibody. The arrowhead indicates the position of MbSrc4-32K.
